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Prolonged survival of Bordetella pertussis in a simple buffer after nasopharyngeal secretion aspiration
S Halperin1, A Kasina, M Swift
1Department of Pediatrics, Dalhousie University, Halifax, N.S., Canada.
Canadian Journal of Microbiology
|November 1, 1992
Abstract:
A simple method for recovery of Bordetella pertussis is described using phosphate-buffered saline containing a casein hydrolysate for transporting secretions collected by nasopharyngeal aspirate. Bordetella pertussis was reisolated from 92% of clinical specimens held at 4 degrees C for 1 week and from all specimens held at -20 degrees C. This method will facilitate the centralization of laboratory facilities for the diagnosis of pertussis.