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Bioprocess factors affecting glycoprotein oligosaccharide structure.
1Department of Chemical Engineering, Stanford University, CA 94305-5025.
Developments in Biological Standardization
|January 1, 1992
Summary
Understanding how bioprocess factors influence glycoprotein oligosaccharide biosynthesis is crucial for developing effective therapeutic and diagnostic glycoprotein products. This review summarizes literature data on these critical bioprocess effects.
Area of Science:
- Biochemistry
- Biotechnology
- Glycobiology
Background:
- Oligosaccharide structures on glycoproteins significantly impact their therapeutic and diagnostic properties.
- Key properties affected include clearance rate, antigenicity, activity, solubility, and stability.
Purpose of the Study:
- To review and summarize existing literature on the influence of bioprocess factors on glycoprotein oligosaccharide biosynthesis.
- To highlight the importance of understanding these influences for glycoprotein product development.
Main Methods:
- Literature review of data from two recent comprehensive reviews.
- Focus on N-linked and O-linked glycosylation pathways.
- Analysis of the effects of cell culture conditions and other bioprocess factors.
Main Results:
- Bioprocess factors demonstrably affect oligosaccharide biosynthesis in glycoproteins.
- Specific conditions can alter glycosylation patterns, impacting critical product attributes.
- Literature data provide a foundation for optimizing bioprocessing.
Conclusions:
- Controlling bioprocess factors is essential for tailoring glycoprotein oligosaccharide structures.
- Optimized glycosylation is key to enhancing the efficacy and safety of glycoprotein therapeutics and diagnostics.
- Further research into bioprocess optimization can lead to improved biopharmaceutical development.