Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phenotypic methods for speciating clinical Aeromonas isolates.

M H Wilcox1, A M Cook, K J Thickett

  • 1Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, Sheffield.

Journal of Clinical Pathology
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

European survey on the current surveillance practices, management guidelines, treatment pathways and heterogeneity of testing of Clostridioides difficile, 2018-2019: results from The Combatting Bacterial Resistance in Europe CDI (COMBACTE-CDI).

The Journal of hospital infection·2022
Same author

Comparison of Whole-Genome Sequence-Based Methods and PCR Ribotyping for Subtyping of Clostridioides difficile.

Journal of clinical microbiology·2021
Same author

Evidence review of physical distancing and partition screens to reduce healthcare acquired SARS-CoV-2.

Infection prevention in practice·2021
Same author

COVID-19: Seroprevalence and Vaccine Responses in UK Dental Care Professionals.

Journal of dental research·2021
Same author

The effect of intestinal microbiota dysbiosis on growth and detection of carbapenemase-producing Enterobacterales within an in vitro gut model.

The Journal of hospital infection·2021
Same author

Protecting the Microbiota.

The Journal of infectious diseases·2021

The suicide phenomenon method accurately speciates clinical Aeromonas isolates in diagnostic labs. Outer membrane protein profiles can aid in identifying Aeromonas caviae and Aeromonas hydrophila.

Area of Science:

  • Clinical microbiology
  • Bacterial speciation
  • Diagnostic laboratory methods

Background:

  • Accurate identification of Aeromonas species is crucial for clinical diagnostics.
  • Phenotypic methods are commonly used for bacterial speciation in clinical laboratories.

Purpose of the Study:

  • To evaluate the suitability of current phenotypic methods for speciation of clinical Aeromonas isolates.
  • To compare the accuracy and efficiency of different Aeromonas speciation schemes.

Main Methods:

  • Comparison of three phenotypic schemes: conventional tests, suicide phenomenon, and API 20 NE with beta haemolysin assay.
  • Analysis of whole cell and outer membrane protein (OMP) profiles using SDS-PAGE for intra-species homogeneity.

Main Results:

Related Experiment Videos

  • The suicide phenomenon scheme showed high agreement (93%) with conventional methods.
  • API 20 NE and the suicide phenomenon concurred for 82% of strains.
  • OMP profiles were useful for identifying Aeromonas caviae (89%) and Aeromonas hydrophila (63%).

Conclusions:

  • The suicide phenomenon method is a simple, accurate, and suitable option for clinical Aeromonas speciation.
  • OMP profiles show potential for confirming Aeromonas caviae and Aeromonas hydrophila identification.