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A colorimetric microassay for the detection of agents that interact with DNA
N S Burres1, A Frigo, R R Rasmussen
1Bioactive Microbial Metabolites, Abbott Laboratories, Abbott Park, Illinois 60064.
Journal of Natural Products
|November 1, 1992
Abstract:
A simple microtiter assay for the detection of compounds that bind DNA is described. Agents that displace methyl green from DNA are detected spectrophotometrically by a decrease in absorbance at 630 nm. The feasibility of using the assay for detecting DNA-active compounds in fermentation extracts was assessed, and the activities of reference compounds in the methyl green assay and an ethidium bromide displacement method were compared.