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Related Experiment Videos

Evaluation of a new automatic calcium ion analyzer.

S Madsen, K Olgaard

    Clinical Chemistry
    |January 1, 1977
    PubMed
    Summary

    The Orion Model SS-20 analyzer offers reliable ionized calcium (Ca2+) measurement in blood and serum. This new system enables routine laboratory testing for Ca2+ with significant advantages.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biomedical Instrumentation

    Background:

    • Ionized calcium (Ca2+) is a critical physiological analyte.
    • Accurate and routine measurement of Ca2+ is essential for clinical diagnostics.
    • Existing methods may have limitations in speed, accuracy, or routine applicability.

    Purpose of the Study:

    • To evaluate the performance of the new Orion Model SS-20 automatic analyzer for ionized calcium (Ca2+) determination.
    • To assess the analyzer's accuracy, precision, and potential interferences in blood and serum samples.
    • To determine the suitability of the Orion SS-20 for routine laboratory use.

    Main Methods:

    • Utilized a flowthrough ion-exchange electrode for Ca2+ measurement.
    • Analyzed heparinized whole blood and serum samples.
    • Investigated within-day and day-to-day variations, analytical recovery, and interference from other ions (K+, Mg2+, Na+).
    • Assessed the impact of pH variations and heparin concentration on Ca2+ readings.
    • Evaluated serum storage effects on Ca2+ measurements.

    Main Results:

    • Demonstrated low within-day (1.2%) and day-to-day (1.6%) variations.
    • Achieved high analytical recovery (92.4%).
    • Identified negligible interference from K+ and Mg2+ within the physiological range.
    • Required correction for sodium error and pH variations (inverse correlation with Ca2+).
    • Found negligible heparin binding effect at minimal concentrations.
    • Observed a decrease in apparent serum Ca2+ after one week of storage at 4°C due to pH increase.
    • Mean values for blood- and serum-Ca2+ (1.10 and 1.07 mmol/liter) were comparable to previous systems.
    • Reported inconveniences related to fluid flow disturbances and electrode malfunction.

    Conclusions:

    • The Orion Model SS-20 provides decisive advantages for ionized calcium (Ca2+) measurement.
    • The analyzer's performance characteristics support its use as a routine laboratory test.
    • Potential interferences (sodium, pH) require appropriate correction for accurate results.
    • Minor technical issues with fluidics and electrodes were noted but did not negate the analyzer's overall utility.

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