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Updated: Aug 7, 2026
A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion
Published on: February 2, 2021
In vitro oxygenation injury to slices prepared from ischemic kidney in rats
1Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Abstract:
When cortical slices prepared from rat kidneys made ischemic were incubated under a 100% oxygen atmosphere, lipid peroxidation increased and the ATP level decreased. Such oxygenation of the slices was accompanied by decreases in gluconeogenesis and the glutathione level, but an antioxidant, N,N'-diphenyl-p-phenylenediamine, prevented the increase in lipid peroxidation without affecting decreases in ATP and glutathione levels, and gluconeogenesis. The results suggest that postischemic oxygenation of slices generates free radicals that cause the production of lipid peroxidation not associated with tissue injury.
Insights
Post-ischemic kidney slices exposed to oxygen showed increased lipid peroxidation and decreased ATP. An antioxidant prevented lipid peroxidation but not other changes, suggesting free radicals cause lipid peroxidation unrelated to tissue injury.
Area of Science:
- Biochemistry
- Renal Physiology
- Oxidative Stress
Background:
- Kidney ischemia can lead to cellular damage.
- Reperfusion injury involves oxidative stress.
- The role of oxygen in post-ischemic kidney slice metabolism is not fully understood.
Purpose of the Study:
- To investigate the effects of post-ischemic oxygenation on rat kidney cortical slices.
- To determine the role of free radicals and lipid peroxidation in this process.
- To assess the impact on cellular energy and metabolic pathways.
Main Methods:
- Preparation of rat kidney cortical slices after induced ischemia.
- Incubation of slices under a 100% oxygen atmosphere.
- Measurement of lipid peroxidation, ATP, glutathione levels, and gluconeogenesis.
- Administration of the antioxidant N,N'-diphenyl-p-phenylenediamine.
Main Results:
- Oxygenation of ischemic kidney slices increased lipid peroxidation and decreased ATP levels.
- Gluconeogenesis and glutathione levels also decreased with oxygenation.
- The antioxidant N,N'-diphenyl-p-phenylenediamine inhibited lipid peroxidation but did not alter ATP or glutathione levels or gluconeogenesis.
Conclusions:
- Post-ischemic oxygenation of rat kidney slices generates free radicals.
- These free radicals induce lipid peroxidation.
- The observed lipid peroxidation is not directly associated with significant tissue injury, as indicated by preserved ATP and glutathione levels and gluconeogenesis.
