Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression and function of matrix metalloproteinases in development.

Z Werb1, C M Alexander, R R Adler

  • 1Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750.

Matrix (Stuttgart, Germany). Supplement
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Research culture, barriers and facilitators within the radiography workforce in the UK - results of a national survey.

Radiography (London, England : 1995)·2025
Same author

Contrasting recruitment of skin-associated adipose depots during cold challenge of mouse and human.

The Journal of physiology·2021
Same author

Evaluation of a personal protective equipment support programme for staff during the COVID-19 pandemic in London.

The Journal of hospital infection·2020
Same author

Evaporative cooling provides a major metabolic energy sink.

Molecular metabolism·2019
Same author

GATA3 targets semaphorin 3B in mammary epithelial cells to suppress breast cancer progression and metastasis.

Oncogene·2017
Same author

Does interhemispheric communication relate to the bilateral function of muscles? A study of scapulothoracic muscles using transcranial magnetic stimulation.

Journal of electromyography and kinesiology : official journal of the International Society of Electrophysiological Kinesiology·2013
Same journal

Studies on purified neutral proteoglycan-degrading enzyme (stromelysin/matrix metalloproteinase 3) from human articular cartilage.

Matrix (Stuttgart, Germany). Supplement·1992
Same journal

The small matrix metalloproteinase of the rat uterus.

Matrix (Stuttgart, Germany). Supplement·1992
Same journal

Literature on vertebrate matrix metalloproteinases and their tissue inhibitors.

Matrix (Stuttgart, Germany). Supplement·1992
Same journal

Nomenclature and glossary of the matrix metalloproteinases.

Matrix (Stuttgart, Germany). Supplement·1992
Same journal

Plasmin activation of collagenase during ovulation in the perfused rat ovary.

Matrix (Stuttgart, Germany). Supplement·1992
Same journal

Inhibition of ovulation in the perfused rat ovary by a synthetic collagenase inhibitor.

Matrix (Stuttgart, Germany). Supplement·1992
See all related articles

Mammalian embryo development involves extracellular matrix remodeling. Metalloproteinases secreted by embryos are regulated and inhibited by TIMP, suggesting their crucial role in implantation and cell interactions.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biochemistry

Background:

  • Extracellular matrix (ECM) remodeling is essential for key developmental processes like cell migration, implantation, and tissue invasion in mammalian embryogenesis.
  • Understanding the molecular mechanisms governing ECM dynamics during early development is critical.

Purpose of the Study:

  • To investigate the expression and function of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) during mouse embryonic development.
  • To elucidate the role of MMPs in trophoblast invasion and cell-ECM interactions.

Main Methods:

  • Analysis of mRNA transcripts for collagenase, stromelysin, and TIMP in mouse embryos.
  • Detection of secreted ECM-degrading metalloproteinases.
  • In vitro studies on the effect of TIMP on parietal endoderm cell migration.

Related Experiment Videos

Main Results:

  • Mouse embryos express and secrete functional collagenase and stromelysin, which are inhibitable by TIMP.
  • Metalloproteinase activity is regulated during peri-implantation development and endoderm differentiation.
  • Trophoblast secretes proteinases during invasion, with reciprocal TIMP expression in the deciduum, suggesting a role in implantation.
  • Exogenous TIMP impacts parietal endoderm cell migration in vitro.

Conclusions:

  • Metalloproteinases are actively involved in ECM remodeling during mammalian embryogenesis.
  • These enzymes, regulated by TIMP, play a significant role in embryonic implantation and cell-ECM interactions.