[Action of O,N'-dichlorodiphenyldichloroethane (O,N'-DDD) on adrenal cortical ATPase]

The action of the inhibitor of the adrenal gland function of dogs--o,n'-DDD--on ATP-ases was studied experimentally. In a concentration of 0.2--1.0 mM o,n'-DDD inhibited the Na+, K+. Mg2+-ATP-ases of the homogenate, and also the mitochondrial and microsomal fractions of the dog adrenal cortex. The effect of the inhibitor became intensified with the temperature elevation. An inhibition of ATP-ases in the homogenate and the microsomal fraction was noted as a result of a single feeding of o,n'-DDD to dogs (50 mg per 1 kg of body weight). A double o,n'-DDD feeding caused activation of the enzymes in the homogenate and the mitochondrial fraction.