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[Detection of inhibitors in swine urine with MICUR-BT]
K Heinritzi1, I Hartel, W Bollwahn
1II. Medizinischen Tierklinik Ludwig-Maximilians-Universität München.
Abstract:
The immersible culture medium MICUR-BT used in human medicine for the detection of antimicrobial substances in the urine was tested for its suitability in pigs. In in-vitro tests, 15 pure antibiotic substances, 2 pure substance mixtures as well as 5 veterinary injection preparations with no direct antimicrobial effect were checked. The agar perforation test was used as a reference procedure. In a total of 65 pigs the detectability of 9 pure antibiotic and chemotherapeutic agents, 2 pure substance mixtures as well as 5 veterinary drugs with no direct antimicrobial effect was tested after oral or parenteral application. In the in-vivo tests the MICUR-BT test turned out to be more reliable than the agar perforation test. In 76.9% of the untreated pigs below 30 kg b.w. the MICUR-BT-test showed an antimicrobial effect. Such effects were reduced by 38.3% in the reference procedure.
Insights
The MICUR-BT test, used in human medicine, effectively detects antimicrobial substances in pigs. This method proved more reliable than the agar perforation test in both in-vitro and in-vivo swine studies.
Area of Science:
- Veterinary Microbiology
- Pharmacology
- Animal Health
Context:
- The detection of antimicrobial residues in food-producing animals is crucial for public health and regulatory compliance.
- Existing methods for antimicrobial detection in swine may have limitations in sensitivity or practicality.
- The MICUR-BT test, a validated tool in human medicine, was evaluated for its potential application in veterinary diagnostics.
Purpose:
- To assess the suitability of the immersible culture medium MICUR-BT for detecting antimicrobial substances in pigs.
- To compare the efficacy of the MICUR-BT test against the agar perforation test in both in-vitro and in-vivo swine models.
- To determine the reliability and sensitivity of the MICUR-BT test for various antimicrobial agents and administration routes in swine.
Summary:
- In vitro testing evaluated 15 pure antibiotic substances, 2 mixtures, and 5 non-antimicrobial veterinary preparations.
- In vivo studies involving 65 pigs assessed the detectability of 9 antibiotics/chemotherapeutics, 2 mixtures, and 5 non-antimicrobial drugs after oral or parenteral application.
- The MICUR-BT test demonstrated higher reliability in vivo compared to the agar perforation test, detecting antimicrobial effects in 76.9% of untreated pigs under 30 kg, versus 38.3% for the reference method.
Impact:
- The MICUR-BT test offers a potentially more reliable and sensitive diagnostic tool for antimicrobial residue detection in swine.
- Findings suggest the MICUR-BT test could enhance veterinary surveillance programs and ensure food safety.
- This study provides a foundation for adopting human diagnostic methods in veterinary medicine for improved animal health management.