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[Isolation and characterization of mutactimycin-producing mutant].

H Li1, W Lu, Y Zhang

  • 1Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|October 1, 1992
PubMed
Summary

UV irradiation of Streptomyces sp. 1254 yielded two mutants producing novel antimicrobial compounds. Mutagenesis activated silent genes, enabling the production of mutactimycins and a water-soluble antibiotic.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Context:

  • Streptomyces species are known for producing a wide array of bioactive compounds, including antibiotics.
  • Strain 1254, a natural non-antibiotic producer, was investigated for its potential to yield novel compounds through mutagenesis.
  • Understanding microbial biosynthesis pathways is crucial for discovering new therapeutic agents.

Purpose:

  • To induce and isolate novel antibiotic-producing mutants from Streptomyces sp. 1254 using UV irradiation.
  • To characterize the chemical and biological properties of the compounds produced by the isolated mutants.
  • To elucidate the genetic basis for the induced antibiotic production.

Summary:

  • UV irradiation of Streptomyces sp. 1254 resulted in two active mutants: mutant 113 producing novel anthracyclines (mutactimycins) with activity against a bacterial virus and tissue culture viruses, and mutant 2-6 synthesizing a water-soluble antimicrobial antibiotic.

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  • Chemical and morphological analyses classified strain 1254 and mutant 2-6 as chemotype I, while mutant 113 was chemotype IV (lacking mycolic acid).
  • Co-synthesis tests and Southern hybridization using the actI gene probe indicated that strain 1254 possesses a silent mutactimycin biosynthesis pathway, which was activated by mutagenesis.

    • Impact:

    • This study demonstrates the potential of mutagenesis in activating silent biosynthetic gene clusters in Streptomyces for novel compound discovery.
    • The identified mutactimycins and the water-soluble antibiotic represent new leads for antimicrobial drug development.
    • The findings contribute to the understanding of Streptomyces secondary metabolite biosynthesis and gene regulation.