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[Determining progesterone in saliva].
H Herges1, W Klinger, U Gethmann
1Frauenklinik, Städtischen Kliniken Osnabrück.
Zentralblatt Fur Gynakologie
|January 1, 1992
Summary
A new radioimmunoassay accurately measures salivary progesterone. However, this method did not clearly show typical progesterone rhythms during the menstrual cycle, suggesting serum measurements may be better for diagnosing infertility.
Area of Science:
- Endocrinology
- Biochemistry
Context:
- Salivary hormone analysis offers a non-invasive method for monitoring endocrine function.
- Accurate measurement of hormones like progesterone is crucial for understanding reproductive health and diagnosing conditions.
Purpose:
- To develop and validate a sensitive radioimmunoassay (RIA) for determining progesterone levels in human saliva.
- To investigate the presence and characteristics of progesterone rhythms in saliva during the normal menstrual cycle and assess circadian variations.
Summary:
- A highly sensitive radioimmunoassay (3.12 pg/ml) was developed for salivary progesterone using a 125I-labelled derivative.
- The study found that biphasic progesterone rhythms were not clearly demonstrated in saliva during the menstrual cycle, with irregular oscillations observed.
- Saliva flow rate did not correlate with progesterone concentration, and serum progesterone measurement appeared more clinically relevant for diagnosing sterility.
Impact:
- Highlights the limitations of salivary progesterone measurements for accurately reflecting cyclical hormonal changes.
- Suggests that serum progesterone analysis remains a more reliable tool for clinical diagnostics, particularly in evaluating infertility.
- Provides a sensitive assay for salivary progesterone, which may still hold value for specific research applications or non-cyclical hormonal assessments.