Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A lactococcal expression system for engineered nisins.

H M Dodd1, N Horn, Z Hao

  • 1AFRC Institute of Food Research, Norwich, United Kingdom.

Applied and Environmental Microbiology
|November 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Linking groundwater chemistry to bovine kidney damage: A one health perspective on fluoride-DOM-hard water interactions in CKDu-hotspots.

Journal of environmental management·2026
Same author

A machine learning model exploring creep performance of dental composites.

Dental materials : official publication of the Academy of Dental Materials·2025
Same author

Intrinsic High-Fidelity Spin Polarization of Charged Vacancies in Hexagonal Boron Nitride.

Physical review letters·2025
Same author

MRI-based bone marrow radiomics for predicting cytogenetic abnormalities in multiple myeloma.

Clinical radiology·2024
Same author

Hidden magnetism uncovered in a charge ordered bilayer kagome material ScV<sub>6</sub>Sn<sub>6</sub>.

Nature communications·2023
Same author

Ethics in pre-ART genetics: a missed X-linked Menkes disease case.

Journal of assisted reproduction and genetics·2023
Same journal

High-throughput viral enumeration of aquatic ecosystems via flow cytometry.

Applied and environmental microbiology·2026
Same journal

Gut microbiota as key mediators of animal acclimation to temperature changes: mechanisms and interventions.

Applied and environmental microbiology·2026
Same journal

Assessing the effects of ocean alkalinity enhancement on marine protozoa: physiological dynamics and transcriptomic responses.

Applied and environmental microbiology·2026
Same journal

The <i>Streptococcus mutans</i> collagen-binding protein Cnm enhances early biofilm formation with <i>Candida albicans</i>.

Applied and environmental microbiology·2026
Same journal

Response of <i>Zostera japonica</i> rhizosphere bacteria to ocean acidification.

Applied and environmental microbiology·2026
Same journal

Dynamics of clinical <i>Klebsiella pneumoniae</i> strains over the COVID-19 pandemic in Qingdao, China.

Applied and environmental microbiology·2026
See all related articles

Researchers engineered Lactococcus lactis to produce novel nisin variants. Modifications created a system for expressing modified nisin, enabling the study of nisin structure-function relationships.

Area of Science:

  • Microbiology and Molecular Biology
  • Bacteriocin Research
  • Genetic Engineering

Background:

  • Lactococcus lactis is a key lactic acid bacterium used in food fermentation.
  • Nisin is a bacteriocin produced by Lactococcus lactis with significant antimicrobial properties.
  • Engineering Lactococcus lactis for enhanced or modified nisin production is of interest for industrial applications.

Purpose of the Study:

  • To develop a Lactococcus lactis strain (FI5876) as an expression system for engineered nisin variants.
  • To investigate the effects of genetic modifications on nisin gene expression and related functions.
  • To produce and characterize novel nisin variants with specific amino acid substitutions.

Main Methods:

  • Genetic modification of the Lactococcus lactis FI5876 strain, including insertional inactivation of the nisA gene.

Related Experiment Videos

  • Characterization of chromosomal rearrangements involving the insertion element IS905.
  • Complementation of the nisA gene lesion using plasmid-encoded genes with site-specific mutations.

    • Main Results:

    • Insertional inactivation of the nisA gene affected downstream genes, including those for nisin immunity.
    • Chromosomal rearrangements led to a strain deficient in native nisA but retaining essential nisin maturation and secretion functions.
    • Plasmid-based complementation with mutated nisA genes resulted in the exclusive production of altered nisin variants.

    Conclusions:

    • The engineered Lactococcus lactis strain serves as a viable expression system for producing modified nisin.
    • Specific amino acid substitutions in nisin can be achieved through targeted genetic engineering.
    • This approach facilitates the study of nisin structure-function relationships and the development of novel bacteriocins.