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[Demonstration of prolactin messenger RNA after amplification in the brain in rats]
J L Valatx1, L Paut-Pagano, M Fevre-Montange
1Laboratoire de Médecine expérimentale, I.N.S.E.R.M. U n. 52, C.N.R.S.-U.R.A. n. 1195, Université Claude-Bernard, Lyon.
Abstract:
By means of immunocytochemistry, a central neuronal network containing a prolactin-like substance has been described in the rat. In order to demonstrate the synthesis of this peptide in these cells, we examined the presence of prolactin messenger RNA (PRL mRNA) in several brain samples including the pituitary gland. Amplification of the PRL mRNA was performed by the polymerase chain reaction technique, followed by southern blotting and hybridization with a specific oligonucleotide. Results showed the presence of the expected cDNA (468 bp) in the hypothalamus. Another cDNA with a lower molecular weight was also observed.