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Related Experiment Videos

Mitotic regulation in Aspergillus nidulans.

N R Morris1, S W James, M J O'Connell

  • 1Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert W. Johnson Medical School, Piscataway 08854.

Ciba Foundation Symposium
|January 1, 1992
PubMed
Summary

The nimA gene promotes mitosis, while bimE inhibits it in Aspergillus nidulans. Disrupting bimE allows mitosis even with DNA replication issues, indicating nimA

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Area of Science:

  • Cell biology
  • Molecular genetics
  • Fungal development

Background:

  • The cell cycle is regulated by intricate molecular pathways.
  • Mitosis regulation involves positive and negative control mechanisms.
  • Aspergillus nidulans serves as a model organism for studying cell cycle control.

Purpose of the Study:

  • To investigate the roles of nimA and bimE genes in regulating mitosis in Aspergillus nidulans.
  • To determine the relationship between nimA, bimE, and the DNA replication checkpoint.
  • To elucidate the requirement of p34cdc2/MPF kinase activation in bimE-mediated mitotic progression.

Main Methods:

  • Genetic analysis of Aspergillus nidulans mutants, including double mutants with temperature-sensitive alleles.
  • Overexpression of nimA gene.
  • Inactivation of bimE gene.
  • Western blot analysis using antibodies against bimE fusion proteins.

Main Results:

  • Overproduction of nimA or inactivation of bimE overrides the DNA replication checkpoint, inducing mitosis.
  • nimA kinase is not essential for chromatin condensation and spindle polymerization when bimE is defective.
  • bimE inactivation requires p34cdc2/MPF kinase activation for chromatin condensation and spindle polymerization.

Conclusions:

  • nimA acts as a positive regulator, and bimE as a negative regulator of mitosis.
  • The bimE-mediated mitotic block is dependent on the activation of the p34cdc2/MPF kinase.
  • These findings provide insights into the complex regulation of the cell cycle and mitosis in eukaryotes.

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