Related Experiment Video
Updated: Feb 28, 2026

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
Published on: July 8, 2025
Metabolic transient studies by NMR
T Binzoni1, G Ferretti, K Schenker
1Dépt. de Physiologie, CMU, Genève, Switzerland.
Abstract:
The time course of phosphocreatine (PC) hydrolysis in humans was measured by 31P-NMR spectroscopy (31P-NMRS) with a time resolution of 10.8 s in the gastrocnemius muscle and a relationship between muscle O2 consumption (VO2) and [PC] was derived from a bioenergetic model. This allowed a direct estimate of the half-time of the intracellular VO2 kinetics (t1/2 VO2) of the contracting human gastrocnemius in aerobic conditions. t1/2 VO2 was found to be approximately 16 s and independent of the work load. This value corresponds to the shortest t1/2 VO2 determined at the mouth of the subject in the absence of lactate accumulation in the rest to work transient. t1/2 VO2 may now be assessed in man at low muscle temperatures. To this aim a procedure was developed allowing corrections of the 31P-NMR spectra based on the muscle temperature profiles obtained by a simultaneously acquired proton image.
More Related Videos
11:02Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
Published on: November 29, 2024
07:54Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
Published on: December 15, 2011
Related Concept Videos
¹H NMR of Labile Protons: Temporal Resolution
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
Applications Of NMR In Biology
Chemical Shift: Internal References and Solvent Effects
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
Double Resonance Techniques: Overview
Spin decoupling is usually achieved by...
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR