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High-performance liquid chromatography-electrochemical detection of 3-methylhistidine in human urine
P Betto1, G Ricciarello, S Pichini
1Istituto Superiore di Sanità, Rome, Italy.
Journal of Chromatography
|December 23, 1992
Abstract:
A reversed-phase high-performance liquid chromatographic method is described for the determination of 3-methylhistidine content in human urine using pre-column derivatization with phenylisothiocyanate, isocratic elution with 15 mM sodium acetate-acetonitrile (92:8, v/v) and electrochemical detection. The limit of quantitation was 0.1 pmol. The method has been applied in routine analyses of 3-methylhistidine in both clinical and research work.