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Transmembrane signaling changes with aging.

T Fülöp1, G Barabás, Z Varga

  • 1First Department of Medicine, University Medical School of Debrecen, Hungary.

Annals of the New York Academy of Sciences
|January 5, 1993
PubMed
Summary
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Aging significantly alters immune cell signaling. Elderly individuals show decreased neutrophil (PMNLs) responses to FMLP and changes in lymphocyte G protein expression, indicating age-related immune dysfunction.

Area of Science:

  • Immunology
  • Cellular Signaling
  • Gerontology

Background:

  • Immune responses and transmembrane signaling pathways are known to change with aging.
  • G proteins play a crucial role in cellular communication and signal transduction.

Purpose of the Study:

  • To characterize G proteins in polymorphonuclear leukocytes (PMNLs) and lymphocytes.
  • To investigate age-related alterations in the amounts and activity of these G proteins.

Main Methods:

  • Studied the effects of formyl-methionyl-leucyl-phenylalanine (FMLP) on PMNLs inositol trisphosphate (IP3) formation.
  • Assessed FMLP receptor binding affinity in young and elderly subjects.
  • Used cholera toxin (CT) and pertussis toxin (PT) for ADP-ribosylation of G proteins in lymphocytes and PMNLs, followed by autoradiography.

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Main Results:

  • Elderly individuals exhibited significantly decreased PMNLs IP3 formation in response to FMLP compared to younger subjects.
  • No significant differences in FMLP receptor binding affinity were observed between age groups.
  • CT labeling revealed age-dependent shifts in G protein expression in lymphocytes, with a prominent 45 kDa polypeptide in the elderly versus a 40 kDa polypeptide in the young.
  • CT labeling in PMNLs showed a stronger 45 kDa band in the elderly and weaker 52/40 kDa bands in the young.
  • PT labeling did not reveal age-related changes but showed cell-type specific differences.

Conclusions:

  • Aging is associated with impaired FMLP-induced signaling in neutrophils.
  • Age-related changes in G protein expression, particularly involving the 45 kDa polypeptide in lymphocytes and PMNLs, may contribute to altered immune function in the elderly.