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In vitro characteristics of Yersinia pseudotuberculosis of nonhuman primate origin
1Deutsches Primatenzentrum GmbH, Göttingen, Germany.
Abstract:
Six strains of serotypes 1 or 2 of Y. pseudotuberculosis were isolated from dead squirrel monkeys, a cotton-top tamarin and a marmoset hybrid. All strains harboured a 71.6 kb plasmid, all were totally oxacillin-resistant and partially resistant to cephalosporins. Biochemically, serotypes 1 and 2 differed from each other in their beta-galactosidase production in a nonfermenter system, whereas the lack of rhamnose, maltose, salicin and trehalose fermentation seemed to be attributable to technical causes.
Insights
Six Yersinia pseudotuberculosis strains (serotypes 1 or 2) were isolated from non-human primates. All strains carried a plasmid and showed resistance to oxacillin and cephalosporins.
Area of Science:
- Veterinary Microbiology
- Primate Health
- Infectious Diseases
Background:
- Yersinia pseudotuberculosis is an important zoonotic pathogen.
- Infections in non-human primates can have significant health and economic impacts.
- Understanding Y. pseudotuberculosis epidemiology in primates is crucial for disease control.
Purpose of the Study:
- To characterize Yersinia pseudotuberculosis strains isolated from deceased non-human primates.
- To investigate the antimicrobial resistance profiles and genetic elements of these isolates.
Main Methods:
- Isolation and serotyping of Y. pseudotuberculosis from primate samples.
- Plasmid analysis to identify genetic determinants.
- Antimicrobial susceptibility testing for oxacillin and cephalosporins.
- Biochemical characterization of Y. pseudotuberculosis serotypes.
Main Results:
- Six strains of Y. pseudotuberculosis (serotypes 1 or 2) were identified in squirrel monkeys, a cotton-top tamarin, and a marmoset hybrid.
- All isolates contained a 71.6 kb plasmid.
- Complete resistance to oxacillin and partial resistance to cephalosporins were observed in all strains.
- Biochemical differences were noted between serotypes 1 and 2 regarding beta-galactosidase production.
Conclusions:
- Yersinia pseudotuberculosis serotypes 1 and 2 can cause mortality in non-human primates.
- The presence of a common plasmid and observed antibiotic resistance patterns warrant further investigation.
- Standardized biochemical testing protocols are recommended for accurate Y. pseudotuberculosis serotyping.