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Characterization of yeast artificial chromosomes containing interleukin genes on human chromosome 5
W Stock1, S C Chandrasekharappa, W L Neuman
1Department of Medicine, University of Chicago, IL 60637.
Cytogenetics and Cell Genetics
|January 1, 1992
Abstract:
To understand better the organization and linkage of the interleukin genes, IL4 and IL5, we prepared long-range restriction maps of five yeast artificial chromosomes (YACs) containing IL5. We determined that IL4 and IL5 are within 100-170 kb, and that the regions surrounding these genes contain several GC-rich areas. Fluorescence in situ chromosomal analysis demonstrated that three of the five YAC clones contain non-contiguous genomic sequences originating from multiple human chromosomes.