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Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation
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Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation

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Two-dimensional gel analysis of histones and other H2AX-related methods

Duane R Pilch1, Christophe Redon, Olga A Sedelnikova

  • 1Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, Maryland 20892, USA.

Methods in Enzymology
|February 12, 2004
PubMed
Abstract

No abstract available in PubMed .

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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...