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Related Experiment Videos

Visible light is able to regulate neurite outgrowth.

Akon Higuchi1, Hanako Kitamura, Ko-Ichi Shishimine

  • 1Department of Applied Chemistry, Seikei University, 3-3-1 Kichijoji-kitamachi, Musashino, Tokyo 180-8633, Japan. higuchi@ch.seikei.ac.jp

Journal of Biomaterials Science. Polymer Edition
|February 12, 2004
PubMed
Summary

Visible light reversibly controls neurite outgrowth in PC12 cells, a neuronal model. Higher light intensities suppress neurite formation, offering potential for patterned neuronal network creation.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biophotonics

Background:

  • PC12 cells, a pheochromocytoma line, exhibit neuronal properties.
  • Nerve growth factor (NGF) induces differentiation and neurite outgrowth in PC12 cells.

Purpose of the Study:

  • To investigate the effect of visible light on PC12 cell neurite outgrowth.
  • To determine if light can be used to control neuronal network formation.

Main Methods:

  • PC12 cells were cultured on polystyrene and extracellular matrix-coated surfaces.
  • Visible light irradiation was applied at varying intensities.
  • Neurite outgrowth was quantified under different light conditions.

Main Results:

  • Neurite outgrowth was reversibly inhibited by visible light in a dose-dependent manner.

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  • Light intensities above 300 Lux (130 microW) significantly suppressed neurite formation.
  • The effect of light depended on the culture substrate.

    • Conclusions:

    • Visible light can be used to control neurite outgrowth in PC12 cells.
    • This technique holds potential for creating patterned neuronal networks using light stimuli.