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Cytotoxicity of composite resins polymerized with different curing methods
A Nalçaci1, M D Oztan, S Yilmaz
1Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, Ankara University, and Sap Enstitüsü, Ankara, Turkey. analcaci@dentistry.ankara.edu.tr
International Endodontic Journal
|February 12, 2004
Summary
This study found that resin composite materials showed cytotoxicity to L 929 cells after 48 hours, but this effect diminished after 7 days. Polymerization curing methods did not significantly alter the cytotoxicity of these dental composites.
Area of Science:
- Biomaterials Science
- Dental Materials
- Cell Biology
Background:
- Resin-based composites are widely used in restorative dentistry.
- Understanding their cytotoxicity is crucial for patient safety.
- Curing methods may influence the release of cytotoxic byproducts.
Purpose of the Study:
- To compare the cytotoxicity of three resin composite materials.
- To evaluate the impact of standard, soft-start, and fast curing methods.
- To assess cytotoxicity on L 929 cells over a 7-day period.
Main Methods:
- Ten discs of Flowline, P 60, and Z 250 composites were prepared.
- Materials were polymerized using standard, soft-start, or fast cure methods.
- Extracts were tested for cytotoxicity on L 929 cells using MTT assay after 1, 2, 3, 5, and 7 days.
Main Results:
- Cytotoxicity was observed at the 2-day interval for most groups.
- Flowline composites cured with Optilux 401 or Elipar Free Light showed no toxicity at 1 and 7 days.
- All tested groups exhibited reduced cell viability compared to the control at 3 and 5 days.
Conclusions:
- Resin composites demonstrated transient cytotoxicity, resolving after 7 days of pre-incubation.
- The polymerization curing method did not significantly influence the cytotoxicity of the tested composite resins.
- Dental composite cytotoxicity is time-dependent and diminishes with aging in biological media.