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Related Experiment Videos

A pseudogene for human glutathione peroxidase.

A M Diamond1, R Cruz, C Bencsics

  • 1Department of Radiation and Cellular Oncology, University of Chicago, IL 60637.

Gene
|December 15, 1992
PubMed
Summary

The glutathione peroxidase (GPx) gene on chromosome 21 is a processed pseudogene, not a functional gene. This finding clarifies the genetic basis of GPx function and evolution.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Biochemistry

Background:

  • Glutathione peroxidases (GPx) are crucial for cellular protection against peroxides.
  • Multiple GPx gene loci exist, but only the chromosome 3 locus was thought to be functional.
  • GPx loci on chromosomes 21 and X lack introns, suggesting they are not functional genes.

Purpose of the Study:

  • To molecularly clone and sequence the GPx locus on human chromosome 21.
  • To determine if the chromosome 21 GPx locus encodes a functional gene.
  • To investigate the evolutionary history of GPx pseudogenes.

Main Methods:

  • Molecular cloning of the GPx locus from human chromosome 21.
  • Nucleotide sequencing of the cloned locus.
  • Comparative sequence analysis with known GPx genes.

Main Results:

  • The nucleotide sequence of the chromosome 21 GPx locus was determined.
  • The locus was identified as a highly conserved processed pseudogene.
  • Intron sequence deletions were confirmed in the chromosome 21 locus.

Conclusions:

  • The GPx locus on chromosome 21 represents a processed pseudogene, not a functional gene.
  • This pseudogene likely arose from a retrotransposed copy of the active GPx gene.
  • The findings support the hypothesis that only the chromosome 3 locus encodes a functional GPx gene.

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