Related Experiment Video
Updated: Aug 27, 2026

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
Published on: May 29, 2016
Structural and functional characterization of the Bacillus megaterium uvrBA locus and generation of UV-sensitive
1Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149 Münster, Germany.
Abstract:
The Bacillus megaterium genes uvrB and uvrA, encoding two subunits of the (A)BC excinuclease, which is responsible for nucleotide excision repair, were isolated and functionally characterized. RNA analyses revealed co-transcription of both genes probably forming a bicistronic operon. Expression of uvrB and uvrA was inducible by the DNA-damaging agent mitomycin C. This finding agrees with the presence of a potential DinR box within the uvrBA promoter. Single inactivation of uvrB or uvrA as well as the parallel knockout of both genes resulted in mutants highly sensitive to UV irradiation. Thus, this locus represents an attractive target for generating biologically safe containment strains of B. megaterium.
More Related Videos
Related Concept Videos
Other Unique Bacteria
Regulation of Bacterial Virulence

