Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytological findings in urothelial carcinoma of the bladder with trophoblastic differentiation.

Cytopathology : official journal of the British Society for Clinical Cytology·2012
Same author

Immunohistochemical expression of keratin proteins in keratoacanthomas of the skin.

Oncology reports·2011
Same author

Immunohistochemical characteristics of breast-carcinoma in patients receiving neuroleptic therapy.

Oncology reports·2011
Same author

Lectin bindings in non-neoplastic esophageal epithelium and esophageal carcinomas.

Oncology reports·2011
Same author

Immunohistochemical profile of phyllodes tumors of the breast.

Oncology reports·2011
Same author

Keratin expression in normal uterine cervical epithelium and carcinomas of cervical origin.

Oncology reports·2011

Related Experiment Video

Updated: Jul 2, 2026

Cell Population Analyses During Skin Carcinogenesis
06:53

Cell Population Analyses During Skin Carcinogenesis

Published on: August 21, 2013

Lectin-binding profiles for normal skin appendages and their tumors.

A Tsubura1, Y Fujita, M Sasaki

  • 1Department of Pathology, Kansai Medical University, Osaka, Japan.

Journal of Cutaneous Pathology
|December 1, 1992
PubMed
Summary

This study maps lectin binding sites in skin appendages and tumors. Findings reveal distinct lectin patterns in eccrine, apocrine, hair follicle, and sebaceous structures, aiding tumor characterization.

More Related Videos

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
05:48

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Published on: March 18, 2014

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections
06:09

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Published on: February 9, 2020

Related Experiment Videos

Last Updated: Jul 2, 2026

Cell Population Analyses During Skin Carcinogenesis
06:53

Cell Population Analyses During Skin Carcinogenesis

Published on: August 21, 2013

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
05:48

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Published on: March 18, 2014

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections
06:09

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Published on: February 9, 2020

Area of Science:

  • Dermatology
  • Histochemistry
  • Oncology

Background:

  • Lectin histochemistry is crucial for characterizing cell surface glycans.
  • Skin appendages (eccrine, apocrine, hair follicles, sebaceous glands) have unique glycan profiles.
  • Understanding these profiles aids in differentiating normal structures from tumors.

Purpose of the Study:

  • To investigate and map lectin-binding sites in normal skin appendages and related tumors.
  • To compare lectin-binding patterns between normal tissues and tumors.
  • To assess the utility of lectins in classifying pilosebaceous and sweat gland tumors.

Main Methods:

  • Histochemical analysis of 60 skin appendage tumors and normal adjacent tissues.
  • Use of four biotinylated lectins: peanut agglutinin (PNA), Dolichos biflorus agglutinin (DBA), soybean agglutinin (SBA), and Ulex europaeus agglutinin-1 (UEA-1).
  • Avidin-biotin-horseradish peroxidase labeling technique for visualization.

Main Results:

  • Distinct lectin staining patterns were observed in eccrine, apocrine, hair follicle, and sebaceous gland components.
  • UEA-1 stained dark cells in eccrine glands; DBA and SBA stained basal cells.
  • Pilosebaceous tumors showed lectin-binding patterns analogous to normal pilosebaceous units, while sweat gland tumors differed from normal tissues.

Conclusions:

  • Lectin binding reveals specific glycan expression in different skin appendage compartments.
  • The distinct patterns can help differentiate between normal and neoplastic tissues.
  • Lectin histochemistry offers a valuable tool for the histopathological classification of skin appendage tumors.