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Immunoelectronmicroscopic study of the nucleoid structure of hydrogen bacteria
O A Mogilnaya1, E V Kiselyova, S E Medvedeva
1Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk.
Journal of Basic Microbiology
|January 1, 1992
Abstract:
Electron microscopical studies of the nucleoid structure of hydrogen bacteria using ultrahin sections and spread DNA from bacterial cell lysates revealed a different DNA packaging in the cell. A compact state of the major part of DNA at all growth stages and stability of nucleosome-like structures were shown. The use of antibodies to HU protein of E. coli labelled by protein A-colloidal gold demonstrated the immunological relationship between HU protein of E. coli and histone-like proteins of Alcaligenes eutrophus and their possible role in the nucleosome-like DNA packaging in procariotic genome.