Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Haemoglobin A2 measurement using high performance liquid chromatography.

R Deacon-Smith1, I Lord

  • 1Department of Haematology, Poole General Hospital, England, UK.

Medical Laboratory Sciences
|June 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multidisciplinary team management is associated with improved outcomes after surgery for esophageal cancer.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2006
Same author

Rapid pre-screening: a validated quality assurance measure in cervical cytology.

Cytopathology : official journal of the British Society for Clinical Cytology·2003
Same author

A new beta chain variant, Hb Tyne [beta 5(A2)Pro-->Ser].

Hemoglobin·1994
Same author

Dietary supplementation and immunocompetence in housebound elderly subjects.

British journal of biomedical science·1994
Same author

The ascorbate cyanide test and the detection of females heterozygous for glucose-6-phosphate dehydrogenase deficiency.

Medical laboratory sciences·1982
Same author

Tympanoplasty in Britain.

The Annals of otology, rhinology, and laryngology·1972
Same journal

Bovine spongiform encephalopathy (BSE): a stimulus to wider research.

Medical laboratory sciences·1992
Same journal

HBsAg vaccinaemia in a newborn.

Medical laboratory sciences·1992
Same journal

Clinical waste disposal.

Medical laboratory sciences·1992
Same journal

Stereomicroscopy in clinical microbiology.

Medical laboratory sciences·1992
Same journal

Diagnostic dilemmas in acutely ill elderly people.

Medical laboratory sciences·1992
Same journal

Principal alterations to drug kinetics and dynamics in the elderly.

Medical laboratory sciences·1992
See all related articles

High-performance liquid chromatography (HPLC) offers a rapid and reliable method for measuring Haemoglobin A2 levels. This technique shows excellent correlation with the ICSH standard, proving effective for diagnosing beta-thalassaemia trait.

Area of Science:

  • Clinical Chemistry
  • Hematology

Background:

  • Accurate measurement of Haemoglobin A2 is crucial for diagnosing beta-thalassaemia trait.
  • Traditional methods can be time-consuming or require specific protocols.

Purpose of the Study:

  • To evaluate the efficacy of a high-performance liquid chromatography (HPLC) system for Haemoglobin A2 quantification.
  • To determine if the protocol for Haemoglobin A1C measurement could be directly applied to Haemoglobin A2 analysis.

Main Methods:

  • Utilized a modular HPLC system with a protocol optimized for Haemoglobin A1C measurement.
  • Compared HPLC results for Haemoglobin A2 with the International Committee for Standardisation in Haematology (ICSH) microchromatography method.

Main Results:

Related Experiment Videos

  • Demonstrated a strong correlation (r = 0.96; P < 0.001) between the HPLC method and the ICSH recommended technique.
  • Established reference ranges: 2.3-3.2% for normal individuals and 4.0-6.7% for individuals with beta-thalassaemia trait.
  • Confirmed that the HPLC system provides quick and reliable Haemoglobin A2 results without protocol modification.

    • Conclusions:

    • The HPLC system is a reliable, rapid, and efficient method for quantifying Haemoglobin A2.
    • The established protocol for Haemoglobin A1C measurement is directly applicable for Haemoglobin A2 assessment.
    • This HPLC method facilitates accurate diagnosis of beta-thalassaemia trait.