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An improved method for quantitative culture of Malassezia furfur
I M Bergbrant1, A Igerud, P Nordin
1Department of Dermatology, University of Gothenburg, Sahlgrenska Hospital, Sweden.
Research in Microbiology
|September 1, 1992
Abstract:
Quantitative culture of Malassezia furfur from clinically healthy skin in 25 individuals was performed with two different methods using contact plates. The best results were obtained when a glucose peptone yeast extract medium, with the addition of milk, Tween-60, glycerol and glycerol monostearate was used. Different techniques for incubation and the reproducibility of this method were evaluated. Incubation can be done in a plastic bag at 32 or 37 degrees C. This new method is simple, the colonies are easy to identify and the counts are high and reliable.