Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorimetric DNA assay for cell growth estimation.

J Rao1, W R Otto

  • 1Histopathology Unit, Imperial Cancer Research Fund, London, United Kingdom.

Analytical Biochemistry
|November 15, 1992
PubMed
Summary

A new DNA content assay using Hoechst 33258 offers sensitive, rapid cell analysis with minimal liquid handling. This method detects as few as 500 human cells and is compatible with [3H]thymidine incorporation studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Primary synovial sarcoma of the vulva: report of a case].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2025
Same author

[Progression and application of circulating tumor DNA in lymphoma].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2024
Same author

[Mucoepidermoid carcinoma of the stomach: report of two cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2024
Same author

High-'n'-dry? A comparison of cannabis and alcohol use in drivers presenting to hospital after a vehicular collision.

Addiction (Abingdon, England)·2023
Same author

Real-world effectiveness of adalimumab in patients with moderate-to-severe hidradenitis suppurativa: the 1-year SOLACE study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Treatment of metastatic placental site trophoblastic tumor with surgery, chemotherapy, immunotherapy and coil embolization of multiple pulmonary arteriovenous fistulate.

Gynecologic oncology reports·2021

Area of Science:

  • Cell biology
  • Biochemistry
  • Molecular biology

Background:

  • Accurate cell proliferation assessment is crucial for understanding biological processes.
  • Existing DNA content assays can be time-consuming and require extensive liquid handling.

Purpose of the Study:

  • To develop a sensitive and rapid DNA content analysis method.
  • To minimize liquid handling requirements in cell growth assays.

Main Methods:

  • Utilized the fluorescent dye Hoechst 33258 for DNA quantification.
  • Developed a growth assay compatible with various cell types, including keratinocytes and mucus-secreting cells.
  • Enabled simultaneous detection of [3H]thymidine incorporation.

Main Results:

  • The assay demonstrates high sensitivity, detecting as few as 500 diploid human cells.
  • Achieved rapid analysis with minimal liquid handling.
  • Confirmed compatibility with [3H]thymidine incorporation, allowing for combined proliferation and DNA synthesis studies.

Conclusions:

  • The developed Hoechst 33258 assay provides a sensitive, rapid, and efficient method for DNA content analysis.
  • This assay is versatile, applicable to diverse cell types and experimental designs.
  • Offers a valuable tool for cell growth and proliferation studies.

Related Experiment Videos