Related Experiment Videos
[Discrepant results with immunohistochemical detection TDx/ADx?]
M Franke1, S Goenechea, F Heinbach
1Institut für Rechtsmedizin, Universität Bonn.
Beitrage Zur Gerichtlichen Medizin
|January 1, 1992
Summary
Immunoassay systems TDx/ADx showed reproducible results, despite initial discrepancies with thin-layer chromatography for drug screenings like amphetamines and cocaine. Further testing confirmed the reliability of these immunoassay methods.
Area of Science:
- Clinical Chemistry
- Forensic Toxicology
- Analytical Chemistry
Background:
- Significant discrepancies (20%) were observed between thin-layer chromatography (TLC) and immunoassay (TDx/ADx) drug screening results over a two-year period.
- These discrepancies involved common drug classes: amphetamines, opiates, benzodiazepines, barbiturates, and cocaine.
- The high rate of disagreement necessitated a thorough investigation into the reliability of the immunoassay systems.
Purpose of the Study:
- To evaluate the compatibility and performance of the TDx and ADx immunoassay systems.
- To determine the detection limits of the TDx and ADx systems.
- To compare immunoassay results with a validated reference method, thin-layer chromatography.
Main Methods:
- Conducted 883 urine sample screenings between January 1989 and December 1990.
- Utilized both TDx and ADx immunoassay systems for drug detection.
- Employed thin-layer chromatography as a comparative method.
- Calculated correlation coefficients to statistically validate the findings.
Main Results:
- Despite initial discrepancies, statistical analysis using correlation coefficients demonstrated that both TDx and ADx systems are reproducible.
- The study confirmed the reliability of the TDx and ADx immunoassay systems for drug screening.
Conclusions:
- The TDx and ADx immunoassay systems provide reproducible results for drug screening.
- While initial discrepancies were noted, the systems' performance was validated through comparative analysis and statistical correlation.