Related Experiment Video
Updated: Sep 30, 2026

Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
Published on: December 7, 2010
Radioimmunoassay for the detection and quantitation of 5-fluorodexoyuridine
Abstract:
A sensitive radioimmunoassay for 5-fluorodeoxyuridine (FdUrd) and 5-fluoro-2'-deoxyuridine 5'-monophosphate has been developed by using antibody induced in rabbits, [3H]FdUrd, and a separation technique with nitrocellulose filters. Antibody specificity was characterized by using compounds chemically related to FdUrd and comparing their effectiveness for displacing [3H]FdUrd from the antibody-combining site. Drug levels in serum samples from patients receiving FdUrd chemotherapy were easily and rapidly determined, and the serum disappearance of the drug was followed by this method. FdUrd was also detected and quantified in urine samples. According to the antiserum used, as little as 1 to 10 pmol FdUrd were measurable.
More Related Videos
09:39Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
08:37Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
Published on: October 22, 2021