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Related Experiment Videos

Creatinine and automatic analysers in relation to icteric specimens.

B G Blijenberg1, E C Liesting, L Zwang

  • 1Department of Clinical Chemistry, Academic Hospital Rotterdam-Dijkzigt, The Netherlands.

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|November 1, 1992
PubMed
Summary

Bilirubin interferes with serum creatine tests on automated analyzers. The Baxter Paramax showed the best performance among the tested instruments, despite all methods exhibiting sensitivity to this interference.

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Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biochemistry

Background:

  • Accurate serum creatine measurement is vital for diagnosing kidney function.
  • Bilirubin is a common endogenous substance that can interfere with clinical assays.
  • Automated analyzers are widely used in clinical laboratories for routine testing.

Purpose of the Study:

  • To evaluate the interference of bilirubin on serum creatine determination.
  • To compare the performance of three different automated analyzers (Technicon Chem-1, DuPont Dimension, Baxter Paramax) in the presence of bilirubin.
  • To identify potential improvements for creatine assays.

Main Methods:

  • Kinetic Jaffé reaction was employed in all three automated analyzers.
  • High-performance liquid chromatography (HPLC) served as the reference method for comparison.

Related Experiment Videos

  • Serum samples with varying bilirubin levels were analyzed.

    • Main Results:

    • All tested automated analyzers demonstrated sensitivity to bilirubin interference.
    • The Baxter Paramax exhibited the most robust performance with the tested specimens.
    • The degree of interference varied among the instruments.

    Conclusions:

    • Bilirubin interference is a significant factor affecting serum creatine assays on automated platforms.
    • The Baxter Paramax offers improved reliability for creatine determination in the presence of bilirubin compared to Technicon Chem-1 and DuPont Dimension.
    • Further method modifications and validation are recommended to mitigate bilirubin interference.