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Determination of human mast cell tryptase by bioluminescence-enhanced two-site immunometric assay
E Schneider1, D Gabrijelcic, R Geiger
1Medor GmbH, Herrsching, Bundesrepublik Deutschland.
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|December 1, 1992
Abstract:
A highly sensitive bioluminescence-enhanced two-site immunometric assay for human mast cell tryptase was developed. The usable range of the standard curve was from 10 ng to 10 micrograms per litre. The intra-assay coefficient of variation of the test was between 8.0 and 12.3%, and the inter-assay coefficient of variation between 8.3 and 13.5%. Recovery of human mast cell tryptase in human serum was 100% (fraction of serum in the test sample: 0.30).