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Locomotion forces generated by a polymorphonuclear leukocyte.

S Usami1, S L Wung, B A Skierczynski

  • 1Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.

Biophysical Journal
|December 1, 1992
PubMed
Summary
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Researchers measured cell locomotion force using a micropipette system. Cell velocity decreased linearly with counter-pressure, stopping at 17 cm H2O, indicating a force of 0.003 dyn.

Area of Science:

  • Cellular Biology
  • Biophysics
  • Mechanobiology

Background:

  • Quantifying cellular forces during active movement is challenging.
  • Understanding cell locomotion mechanics is crucial for various biological processes.

Purpose of the Study:

  • To directly measure the physical forces generated by cells during active migration.
  • To investigate the relationship between applied counter-pressure and cell velocity.

Main Methods:

  • A specialized micropipette system was developed to observe and manipulate cell movement.
  • Counter-pressure was applied to oppose chemotactic cell migration, allowing for force measurement.

Main Results:

  • Cell velocity was measured at an average of 0.33 microns/s without counter-pressure.

Related Experiment Videos

  • Increasing counter-pressure linearly decreased cell velocity.
  • Cell locomotion ceased at 17 cm H2O counter-pressure, corresponding to 0.003 dyn.

    • Conclusions:

    • The study provides a direct method for measuring cellular locomotion force.
    • Cellular movement force is quantifiable and directly related to applied opposing pressure.
    • Maximum cellular work rate was estimated at 2.5 x 10(-8) erg/s.