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Cloning of DNA from a Rhodococcus strain conferring the ability to decolorize sulfonated azo dyes
G S Heiss1, B Gowan, E R Dabbs
1Department of Genetics, University of the Witwatersrand, Johannesburg, South Africa.
FEMS Microbiology Letters
|December 1, 1992
Abstract:
Azo dyes are recalcitrant pollutants. Two sulfonated azo dyes, Orange II and Amido black, are effectively decolorized by certain nocardioform strains of the genus Rhodococcus. A mutant of one of these strains was isolated which had lost azo-dye decolorizing ability and the strain was used to clone DNA conferring this ability, by screening a BclI library constructed from DNA of a decolorizing strain. The relevant genetic information was located on a 6.3-kb fragment of DNA.