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Ion-pair high-performance liquid chromatography of diaminopimelic acid in hydrolysates of physiological samples
M E Dugan1, W C Sauer, K A Lien
1Department of Animal Science, University of Alberta, Edmonton, Canada.
Journal of Chromatography
|November 6, 1992
Abstract:
A method is reported for the determination of diaminopimelic acid (DAPA) in physiological samples. DAPA is derivatized with an o-phthaldialdehyde reagent solution, subjected to reversed-phase high-performance liquid chromatography and detected spectrofluorometrically. The method is a significant advance over previous methods because it uses the ion-pairing agent hexadecyltrimethylammonium bromide (HTMA) to facilitate DAPA measurement. Ion-pairing with HTMA avoids interference with co-eluting derivatives to provide simultaneous, sensitive, reproducible measurement of both DAPA peaks (DD,LL-DAPA and DL-DAPA).