Related Experiment Video
Updated: Jul 5, 2026
12:21
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
Published on: November 30, 2013
[In situ hybridization histochemistry for the kidney diseases]
1Dept. of Biological Science and Technology, Faculty of Engineering, University of Tokushima.
Nihon Rinsho. Japanese Journal of Clinical Medicine
|December 1, 1992
Summary
In situ hybridization reveals molecular changes in kidney disease, examining gene expression during renal regeneration and ischemic injury. This study proposes a probe bank to advance kidney disease research.
Area of Science:
- Molecular biology
- Nephrology
- Genetics
Context:
- In situ hybridization (ISH) is a powerful technique for visualizing gene expression within tissue samples.
- Kidney diseases affect millions globally, necessitating advanced diagnostic and research tools.
- Understanding molecular mechanisms is key to developing targeted therapies for renal pathologies.
Purpose:
- To highlight critical aspects of in situ hybridization for studying kidney diseases at the molecular level.
- To present examples of ISH applications in renal research, including gene expression during regeneration and injury.
- To propose the establishment of a probe bank to facilitate future ISH studies in nephrology.
Summary:
- This study details crucial points of in situ hybridization (ISH) methods for investigating kidney diseases.
- Two examples illustrate ISH applications: analyzing hepatocyte growth factor gene expression in renal regeneration post-nephrectomy and metallothionein gene induction in ischemic acute renal failure.
- The authors suggest creating a dedicated probe bank to support and standardize ISH research in nephrology.
Impact:
- Provides a methodological overview of ISH for kidney disease research.
- Demonstrates the utility of ISH in understanding gene regulation during renal recovery and disease states.
- Lays the groundwork for a centralized resource (probe bank) to enhance the efficiency and reproducibility of molecular studies in nephrology.
Related Concept Videos
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
01:12Internal Anatomy of the Kidney
The kidneys are essential organs in the human body, performing a myriad of tasks that maintain homeostasis and overall health.
Anatomical Position and Dimensions
The kidneys are retroperitoneal organs positioned against the posterior abdominal wall on either side of the spine, roughly between the twelfth thoracic and third lumbar vertebrae. Each kidney is typically 10-12 cm long, 5-6 cm wide, and 3-4 cm thick, weighing about 150 grams.
Renal Cortex
The outermost region of the kidney is the...
Anatomical Position and Dimensions
The kidneys are retroperitoneal organs positioned against the posterior abdominal wall on either side of the spine, roughly between the twelfth thoracic and third lumbar vertebrae. Each kidney is typically 10-12 cm long, 5-6 cm wide, and 3-4 cm thick, weighing about 150 grams.
Renal Cortex
The outermost region of the kidney is the...
01:28Kidney Transplant I: Introduction
A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
01:30Acute Kidney Injury IV: Diagnostic Studies and Prevention
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
01:25Chronic Kidney Disease I: Introduction
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...