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Progressive changes in cytotoxic potential during mixed lymphocyte culture.
Journal of Immunology (Baltimore, Md. : 1950)
|May 1, 1978
Summary
Cytotoxic function in mouse lymphocytes requires DNA synthesis for initial expression. Subsequent cell divisions can alter the cytotoxicity per cell, indicating this potential is dynamic, not static.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Initial cytotoxic function in mouse lymphocytes responding to alloantigen in vitro requires at least one round of DNA synthesis.
- The role of subsequent cell divisions in maintaining or altering cytotoxic function remains unclear.
Purpose of the Study:
- To determine if further cell divisions are solely for clonal expansion or if they modify individual cytotoxic cell potential.
- To investigate the dynamic changes in cytotoxicity per cell during an immune response.
Main Methods:
- Comparison of cytotoxicity levels per cell count at different culture stages.
- Estimation of cytotoxic cell frequency using effector-target cell conjugates.
Main Results:
- Cytotoxicity per cell is not a fixed attribute.
- The cytotoxic potential of cells can be modulated (increased or decreased) during the immune reaction.
Conclusions:
- Cell division beyond the initial requirement can dynamically alter the cytotoxic capacity of lymphocytes.
- Cytotoxic potential is a modifiable property influenced by the cellular environment and progression of the immune response.