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Direct sequencing and bidirectional allele specific polymerase chain reaction of the bovine beta-casein B variant

G Damiani1, F Pilla, P Leone

  • 1Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, CNR, Milano, Italy.

Animal Genetics
|January 1, 1992
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...