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[The use of tapioca as coverage in viral titration]
P R Post1, R de Carvalho, E C Brito
1Departamento de Bioquímica e Biologia Molecular, Instituto de Tecnologia em Imunobiológicos, Rio de Janeiro, Brasil.
Anais Da Academia Brasileira De Ciencias
|January 1, 1992
Summary
Tapioca can be used as a safe and effective overlay medium for viral titration, specifically for Yellow Fever virus (YF) and Measles virus. This study found Tapioca yielded higher viral titers compared to Karaya gum.
Area of Science:
- Virology
- Cell Biology
- Vaccine Quality Control
Background:
- Viral titration is crucial for vaccine quality control.
- Plaque assays commonly use overlay media for viral titrations.
- Evaluating alternative, cost-effective overlay media is beneficial.
Purpose of the Study:
- To evaluate Tapioca as a novel overlay medium for viral titration.
- To assess the toxicity and efficacy of Tapioca compared to conventional media.
- To determine Tapioca's suitability for various viral assays.
Main Methods:
- Toxicity of Tapioca was assessed on Vero cells with and without Yellow Fever virus (YF) 17DD.
- Viral titration of YF 17DD batches was performed using Tapioca and Karaya gum overlays.
- Measles virus titration was conducted using Tapioca overlay on Vero cells.
Main Results:
- Tapioca demonstrated no toxicity to Vero cells, even when infected with YF 17DD.
- Higher viral titers were consistently obtained with Tapioca compared to Karaya gum.
- Tapioca proved effective for thermostability and plaque reduction neutralization tests.
- Measles virus was successfully titrated using Tapioca on Vero cells.
Conclusions:
- Tapioca is a safe, reliable, and cost-effective alternative overlay medium for viral titration.
- Its application extends to YF 17DD, Measles virus, and other viral assays.
- Tapioca offers advantages in viral quantification and vaccine quality control.