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Published on: June 15, 2018
Binding of human IgA to HCl-extracted c protein from group B streptococci (GBS)
A I Kvam1, O J Iversen, L Bevanger
1Department of Microbiology, Faculty of Medicine, Regional Hospital, University of Trondheim, Norway.
Abstract:
The c beta protein of group B streptococci obtained by HCl extraction appears as a ladder-like pattern in SDS-PAGE when detected by a rabbit anti-c beta serum, and a similar picture is seen when the crude extract is incubated with human IgA and an anti-human IgA conjugate. Affinity-purified c beta antigen and IgA receptors from GBS gave identical pictures in Western blots using rabbit anti-c beta serum. Both the c beta antigen and the IgA receptor are exposed on the surface of GBS as demonstrated by immunofluorescence.
