[Effect of vitamins PP and B1 on pentosephosphate pathway enzymatic activity in the kidneys of adrenalectomized rats]

The activity of glucose-6-phosphate dehydrogenase (EC 1.1.1.44), transketolase (EC 1.1.1.44), transketolase (EC 1.1.1.49), 6-phosphogluconate dehydrogenase (EC 2.2.1.1.) and also the thiaminediphosphate (TDP) content were investigated on the 8th day after adrenalectomy. With compensated sodium dysbalance the removal of suprarenals is shown not to change the activity of dehydrogenases, but it does lower the transketolase activity and the TDP level. A seven-fold administration to adrenalectomized animals of nicotinic acid, thiamine or a simple injection of nicotinamide normalize the transketolase activity through raising the TDP level. The fact of the reduced transketolase activity in the kidneys in hypocorticotism being due to deficiency of the co-enzyme is also proved by the presence of the TDP-effect in vitro.