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Ultrastructural studies on hydantoin induced lymphomas in mice
Zeitschrift Fur Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology
|May 31, 1978
Summary
Chronic diphenylhydantoin exposure induced thymic lymphomas in mice, affecting immune systems and leading to abnormal cell changes. Murine C-type particles were observed in cultured lymphoma cells, suggesting a role in disease enhancement.
Area of Science:
- Oncology
- Immunology
- Toxicology
Background:
- Diphenylhydantoin (DPH) is an anticonvulsant drug.
- Chronic exposure to DPH can induce tumors, particularly lymphomas.
- Susceptibility to DPH-induced lymphomas varies among mouse strains.
Purpose of the Study:
- To investigate the ultrastructural changes in mouse thymic lymphomas induced by DPH.
- To explore the relationship between DPH exposure, immune system disturbance, and lymphoma development.
- To identify potential etiological factors, such as viral particles, in DPH-induced lymphomas.
Main Methods:
- Ultrastructural analysis of cells from original tumors and short-term cultures.
- Experimental induction of thymic lymphomas in mice with varying genetic susceptibility.
- Morphological characterization of tumor cells.
Main Results:
- DPH induced thymic lymphomas in susceptible mouse strains (C57Bl, SJL/J) but not in resistant strains (C3Hf).
- Lymphoma development was preceded by lymphoreticular atrophy and reticulohistiocytic hyperplasia.
- Abnormal cellular changes and murine C-type particles were observed in cultured lymphoma cells.
Conclusions:
- Diphenylhydantoin can induce lymphoblastic lymphomas in mice.
- DPH-induced lymphomas are associated with immune system disturbances.
- The presence of C-type particles in cultured cells may contribute to lymphoma enhancement.