[Methods of detecting and determining nitrogen-containing mycotoxins].

Area of Science:

• Analytical Chemistry
• Food Safety
• Mycotoxicology

Context:

• Nitrogen-containing mycotoxins (NM) pose a significant risk to food and feed safety.
• Accurate determination of NM is crucial for regulatory compliance and public health.
• Various analytical challenges exist in the extraction, cleanup, and determination of NM.

Purpose:

• To review and compare different analytical methodologies for the detection and quantification of N-containing mycotoxins (NM).
• To assess the suitability of various nitrogen functional groups for extraction and cleanup procedures.
• To provide an overview of common and emerging techniques for NM analysis in complex matrices.

Summary:

• Thin-layer chromatography (TLC) and liquid chromatography (LC) are widely employed for NM detection and determination.
• Gas chromatography (GC) is utilized for specific NM and their degradation products, often requiring derivatization.
• Immunochemical methods like ELISA are limited to a few NM, such as Ochratoxin A, for which numerous methods exist.

Impact:

• Informs the selection of appropriate analytical methods for NM testing in food and feed industries.
• Contributes to the development of more sensitive and reliable methods for mycotoxin analysis.
• Supports efforts to ensure the safety and quality of the global food supply chain.