Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Experimental postoperative endophthalmitis.

R K Forster1

  • 1King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia.

Transactions of the American Ophthalmological Society
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recurrent fungal keratitis and endophthalmitis.

Cornea·2000
Same author

Nontuberculous mycobacterial keratitis in south Florida.

Ophthalmology·1998
Same author

Conrad Berens Lecture. The management of infectious keratitis as we approach the 21st century.

The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc·1998
Same author

Mutation hot spots in 5q31-linked corneal dystrophies.

American journal of human genetics·1998
Same author

Clinical evidence for hepatitis B transmission resulting from corneal transplantation.

Cornea·1997
Same author

A comparison of two selective interrupted suture removal techniques for control of post keratoplasty astigmatism.

Transactions of the American Ophthalmological Society·1997
Same journal

The Utility of Color Duplex Ultrasonography in the Diagnosis of Giant Cell Arteritis: A Prospective, Masked Study. (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2018
Same journal

Autoimmune Retinopathy: Current Concepts and Practices (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2018
Same journal

Erratum: Predictors of Intraocular Pressure After Phacoemulsification in Primary Open-Angle Glaucoma Eyes with Wide Versus Narrower Angles (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2018
Same journal

The Relationship Between Ocular Itch, Ocular Pain, and Dry Eye Symptoms (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2018
Same journal

The Effects of Phacoemulsification and Intraocular Lens Implantation on Anatomical and Functional Parameters in Patients with Primary Angle Closure: A Prospective Study. (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2017
Same journal

Predictors of Intraocular Pressure After Phacoemulsification in Primary Open-Angle Glaucoma Eyes with Wide Versus Narrower Angles (An American Ophthalmological Society Thesis).

Transactions of the American Ophthalmological Society·2017
See all related articles

Vancomycin treatment did not significantly alter the inflammatory response in an aphakic rabbit model of bacterial endophthalmitis. Different bacterial species showed varied severity and treatment responses, necessitating further research for effective endophthalmitis management.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Infectious Diseases

Background:

  • Bacterial endophthalmitis is a severe intraocular infection that can lead to vision loss.
  • Understanding the differential pathogenicity and treatment responses of common bacterial isolates is crucial for effective management.
  • An aphakic rabbit model provides a platform to study the inflammatory and microbiological aspects of endophthalmitis.

Purpose of the Study:

  • To evaluate the severity of endophthalmitis caused by different bacterial inocula in an aphakic rabbit model.
  • To assess the impact of vancomycin treatment and vitrectomy on the inflammatory response and bacterial killing.
  • To correlate in vitro and in vivo behaviors of bacterial isolates in the context of endophthalmitis.

Main Methods:

  • Intravitreal inoculation of various concentrations of *E. faecalis*, *S. aureus*, *S. epidermidis*, and *B. cereus* into aphakic rabbit eyes.

Related Experiment Videos

  • Clinical and histopathological scoring to assess the severity of inflammation and tissue damage.
  • In vitro susceptibility testing and in vivo bacterial killing assays with vancomycin, with and without vitrectomy.

    • Main Results:

    • Bacterial severity in the absence of treatment ranked: *S. epidermidis* < *B. cereus* < *E. faecalis* < *S. aureus*.
    • High inoculum (10(7) cfu) of *S. epidermidis* and *B. cereus* induced severity comparable to *E. faecalis* and *S. aureus*.
    • Vancomycin treatment, with or without vitrectomy, did not significantly alter the overall inflammatory response.
    • *B. cereus* and *S. epidermidis* showed significant bacterial killing with treatment, while *E. faecalis* and *S. aureus* did not achieve 99.9% killing.
    • No single in vitro result predicted in vivo microbiologic behavior.

    Conclusions:

    • The severity of bacterial endophthalmitis varies among common isolates and is inoculum-dependent.
    • Current vancomycin treatment regimens and vitrectomy showed limited impact on the overall inflammatory response in this model.
    • Further research into anti-inflammatory agents, combination therapies, and optimized treatment strategies is necessary for improved endophthalmitis outcomes.