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Ultrasonically induced in vitro cell lysis: node-antinode interactions.

Y Doida¹, A A Brayman, M W Miller

¹Department of Biology, Shiga University of Medical Science, Japan.

Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine

|August 1, 1992

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Ultrasonic cell lysis in a rotating tube was investigated. Both standing wave and bubble recycling mechanisms contribute to cell damage, with rotation significantly enhancing lysis.

Area of Science:

Biophysics
Acoustic cavitation
Cell biology

Background:

Understanding the mechanisms of ultrasonically induced cell lysis is crucial for applications in cell disruption and drug delivery.
Two primary hypotheses, standing wave effects and bubble recycling, have been proposed to explain cell lysis during sonication.

Purpose of the Study:

To differentiate between the standing wave and bubble recycling hypotheses for ultrasonically induced cell lysis.
To investigate the effect of tube movement and rotation on cell lysis during sonication.

Main Methods:

P-388 cells in aqueous suspension were subjected to sonication (1 MHz, 5 W/cm2, continuous wave, 5 min) in a rotating tube.
Tube movement included back-and-forth oscillation (+/- 3 or +/- 7 mm) and partial or complete rotation.

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Cell lysis was quantified as a measure of the sonication effect.

Main Results:

Linear back-and-forth movement of the tube resulted in approximately 20% cell lysis.
Partial or complete rotation of the tube during sonication led to considerably higher cell lysis (approximately 60%).

Conclusions:

The findings suggest that both standing wave effects and bubble recycling mechanisms play a role in ultrasonically induced cell lysis.
Tube rotation significantly enhances cell lysis, indicating the importance of cavitation dynamics in the process.