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High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
High-throughput expression and purification of 6xHis-tagged proteins in a 96-well format
Jutta Drees1, Jason Smith, Frank Schäfer
1Qiagen, Hilden, Germany.
Methods in Molecular Medicine
|February 13, 2004
Abstract:
By using automation and affinity-tag technologies, analysis of the large number of ORFs generated by genome-sequencing projects is greatly accelerated. Protocols describing culture of E. coli in automation-compatible formats and subsequent micro-to large-scale automated purification of 6xHis-tagged proteins are presented.
