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Updated: Aug 14, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
The budding yeast HRR25 gene product is a casein kinase I isoform
A J DeMaggio1, R A Lindberg, T Hunter
1Molecular Biology and Virology Laboratory, Salk Institute, La Jolla, CA 92037.
Abstract:
The Saccharomyces cerevisiae HRR25 gene was identified as a regulator of DNA strand-break repair. HRR25 encodes a protein kinase that is closely related to bovine casein kinase I (CKI). CKI is a ubiquitous multipotential protein kinase. Rabbit polyclonal antibodies that recognize and immunoprecipitate Hrr25p have been generated and an immune complex protein kinase assay has been developed. The reaction depends upon HRR25 and shows that Hrr25p uses casein as a substrate. The identity between Hrr25p and bovine CKI suggests that Hrr25p is a yeast isoform of the CKI family and that CKIs may play a role in regulating DNA metabolism.
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