Related Experiment Video
Updated: Aug 26, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
Published on: January 9, 2017
Validating the assumption to the interference approximation by use of measurements of absorption efficiency and
Yingqing Huang1, Eva M Sevick-Muraca
1Photon Migration Laboratories, Texas A&M University, Mailstop 3573, College Station, Texas 77843-3573, USA.
Abstract:
Frequency domain photon migration (FDPM) measurements were employed to accurately quantify optical properties of both the suspending fluid and particles within dense polystyrene suspensions of 143- or 226-nm mean diameter at varying concentrations (5-30% by volume). The measured absorption coefficients varied linearly with particle volume fraction whereas the isotropic scattering coefficients varied nonlinearly in agreement with the prediction that utilizes the hard-sphere structure factor model. These results validate the interference approximation of light scattering to describe light propagation accurately within dense suspensions. Furthermore, owing to the accuracy of FDPM absorption measurements, the imaginary refractive indices for both particles and their suspending fluid were determined and were found to compare favorably with literature values.
Related Concept Videos
Atomic Absorption Spectroscopy: Interference
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
UV–Vis Spectroscopy: Beer–Lambert Law
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

