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Updated: Aug 26, 2026
Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Evaluation of a RAPD-based typing scheme in a molecular epidemiology study of Vibrio cholerae O1, Brazil
N C Leal1, M Sobreira, T C Leal-Balbino
1Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, Pernambuco, Brazil.
Aims:
To evaluate the utility of random amplification of polymorphic DNA (RAPD) technique for routine practice in public health laboratories for epidemiological studies of Vibrio cholerae O1 isolates.
Materials And Results:
Seventy-nine strains were examined by PCR for the toxin genes (ctx A, zot and ace), virulence-associated genes (tcp A and tox T) and RAPD sequences. Except for one strain (no. 1123) from the Amazonas State, all the strains analysed carried the genes ctx A, zot, ace, tcp A and tox T. RAPD fingerprinting revealed variability but no correlation with serotype, biotype or geographical origin of the isolates was found.
Conclusion:
A standardized RAPD method does not enable the establishment of a pattern data bank for the identification of V. cholerae O1 strains.
Significance And Impact Of The Study:
The simplicity and discriminative capacity of this technique make it useful for detecting genetic diversity among micro-organisms from a defined group or for outbreak investigation.
